Users on social media were quick to point out the bug that appeared to have no intention of leaving Pence's silver hair as he debated his Democratic challenger Sen. Kamala Harris, D-Calif., at the University of Utah in Salt Lake City.
"The Chinese have clearly planted a bug on Pence!" one user wrote.
"Not me swatting my TV before realizing the fly was on Mike Pence’s hair," another said.
"Congratulations to the fly on Mike Pence's head for experiencing more human contact than I have since March," another joked.
Pence didn’t seem to notice the bug that made a cameo more than halfway through the debate and hung around for about two minutes.
Others would later join in on the fun and post their own fly jokes.
"Give that fly a mail-in ballot," wrote White House adviser Kellyanne Conway.
Comedian Tim Young wrote: "I'm not saying Mike Pence is killing it tonight... but there's an actual dead fly stuck in his hair."
"The deep state planted a bug on @VP. The illegal spying is really out of control," added Sen. Rand Paul, R-Ky.
As the debate was ending, it seemed dozens of people had already created their own Fly on Mike Pence Twitter accounts.
Joe Biden later tweeted, "Pitch in $5 to help this campaign fly," along with the link "flywillvote.com" which redirected to iwillvote.com.
A "Truth Over Flies Fly Swatter" was also being sold for $10 on the Biden campaign website under the new arrivals section.
"I don’t know who needs to hear this but...if your “gotcha” tweet of the night was dedicated to a fly on Mike Pence’s head, it’s because your team lost— Badly," wrote Candace Owens.
After the debate was over, Snapchat allegedly made a Mike Pence white hair with fly filter available, according to journalist Yashar Ali.
The insect eventually buzzed off.